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KMID : 0379120110390010011
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Korean Journal of Mycology
2011 Volume.39 No. 1 p.11 ~ p.15
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Genetic Relationship between Korean Verticillium dahliae Isolates and the Other Verticillium Species
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Shang Fei
Choi You-Ri
Song Jeong-Young
Kim Hong-GI
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Abstract
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To provide basic information for Verticillium spp., molecular methods were applied to analyze genetic characteristics within Verticillium spp. including Verticillium dahliae, isolated from diseased plants in two regions of Korea. Five Korean isolates of V. dahliae causing Verticillium wilt on chrysanthemum were analyzed, together with six other Verticillium spp., using mitochondrial small subunit rRNA gene (rns) sequence and random-amplified polymorphic DNA (RAPD). In a phylogenetic tree based on rns region sequences, Korean V. dahliae isolates formed a single clade with foreign isolates, whereas the other Verticillium spp. formed separate groups. In addition to rns sequence analysis, a dendrogram based on RAPD fragment patterns also showed clustering of all V. dahliae isolates into one group, separate from the six different Verticillium spp., and the V. dahliae isolates formed three subgroups which corresponded to the regions of origin, Kumi, Busan city and Canada. This indicates that high genetic variation exists between regions, although the fungus was isolated from the same host plant, chrysanthemum. These results provide the foundation for the study of genetic diversity and relationships among V. dahliae isolates in Korea.
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KEYWORD
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Genetic relationship, Mitochondrial small subunit rRNA gene (rns), Phylogeny analysis, RAPD, Verticillium dahliae
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